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Frequently Asked Questions

We accept total RNA, genomic DNA, amplicon, cell cultures and pellets, tissues, blood, etc.

GeneInsight do use a state-of-the-art Next-Generation Sequencing (NGS) facility, which houses
cutting-edge genome sequencing platforms such as Illumina’s NovaSeq X plus, long read sequencing
platform PacBio(Revio/Onso), etc. Our Sequencing facility is supported PCR, Sanger Sequencing to
offer comprehensive diagnostic solutions to individual customers, researchers, doctors, and
hospitals.

Next-generation sequencing (NGS) is a sequencing technology that offers ultra-high throughput,
scalability, and faster DNA and RNA sequencing.

The genome is the entire set of DNA instructions found in a cell.

Please send an email to Project Support <projectsupport@geneinsight.ai> with your contact
information. Our sales representative in your region will contact you to take things forward.

Yes, we will be happy to help you. Our sales representative will contact you to understand your
preliminary project requirements. He/She will help (if needed) to have a discussion with a technical
expert (Application Scientists with expertise in the relevant area) to give critical inputs to your
experimental design. Our support team will also arrange a discussion with our team of experts
(Scientists from Wet lab and Bioinformatics) before the project initiation, to review the experimental
design so that your aim & goals are aligned with the deliverables promised in the proposal.

Our sales representative/BD person will provide a proposal after having the initial discussion where
your requirements are finalized.

You may send samples to us in several batches. This will allow us to run QC first to check for the
sample quality. If sample does not pass QC, this will give you some time to prepare new samples, and
we will do the sequencing when we receive all the samples.

For RNA-seq, Trizol is more commonly used, you can just use the Chloroform/Phenol method for
purification as instructed in the Trizol kit. Another easier method is to use the commercial kit (e.g.
Qiagen RNeasy Mini Kit). Moreover, for isolating total RNA from plant samples, we would
recommend Qiagen RNeasy Plant Mini Kit.
For miRNA sequencing, you may isolate your RNA sample with a mirVana™ miRNA Isolation Kit or
Qiagen’s kit. We prefer receiving Total RNA sample however so we can perform the Bioanalyzer QC to
check the RNA quality before beginning library construction.
For other services, there are generally no preferred DNA/RNA isolation kits as long as minimum
requirements for QC are met.

By using proper shipping approaches, the transport will not affect the sample condition. For DNA
samples, most DNA are stable in ice for a few days, the dry ice will not require. For RNA samples,
RNA is much less stable than DNA, you can send with dry ice, with ethanol precipitate or using
RNAstable reagent. For tissue sample, you can send in RNAlater reagents and ship with room
temperature.
Please seal each vial tube to absolutely prevent leakage and breakage and evaporation, and a
secondary container is recommended.

Yes. For WES, we can use degraded DNA. For degraded FFPE RNA, we use Ribo-Zero technology to
deplete rRNA, as the normal protocol will not work due to loss of the poly-A tails.

Due to limited space in the DNA Sequencing facility freezer we only keep your samples for 3 months,
after which time they will be discarded without warning. If you would like your remaining samples
returned to you please contact us, we will charge shipping/handling fee.

Technology Platform

We have the Illumina sequencing platforms(NovoSeq X plus) and PacBio SMRT
Sequel sequencing platform(Revio/Onso), and we are setting up Sanger
Sequencing platform for confirmatory test.

We will provide customers with the QC report in every major steps, i.e. QC of
the initial input sample, library preparation QC and raw data QC to detect the
sequencing quality. Nevertheless, be ensure to do your own quality control
before you ship the samples in order to avoid delays or other pitfalls in
processing.
To monitor sequencing quality control, the PhiX Control will be used. Illumina
PhiX Control can be used as a positive control in the clustering process. If a
problem occurs in sample preparation, PhiX still generates clusters. These
clusters help to discern whether a lack of clusters is due to sample preparation
failure or a failure in the cluster generation process. And we will repeat the
sequencing step if the failure is due to the sequencing run

Sample pricing criteria depends on the assignments involved: service type, project type, number of samples, data required and mode of data transfer

Please email to bdgenetics@geneinsights.ai for payment terms

Standard deliverables in a Sequencing data only project are Sample/Library QC reports, Sequenced
Raw Data,Data QC report. Standard deliverables in a bioinformatics analysis alone project is data
analysis report (Annexture-1). For any additional details for advanced analysis or customized analysis,
you can contact on Project Support projectsupport@geneinsight.ai

Our sale representative in your region will guide you with the shipping of samples. Please refer to our (Annexure 2) for details about shipment protocols.

Our Project support team will acknowledge receipt of your samples over email once they reach our
laboratory. Our support team will create a unique project id for your project and share the details
with you for tracking the project progress (Figure 1: Overview of the Process from lead to receiving of
samples in GeneInsight Informatics Pvt. Ltd).

Our support team will share reports on completion of each milestone such as quality control
(Sample/Library/Sequencing data), sequencing data & analysis. You will be contacted if your
feedback & confirmation is needed to proceed from one milestone step to another.

Timelines vary depending on the project requirement. Standard TAT for Data only projects are
maximum30-45 days and Data + Analysis (Standard) projects are 90 days. TAT will be counted after
samples pass there required QC parameters for processing

We have experts with extensive experience with challenging samples and have many low-input
protocols in place and our Project support team projectsupport@geneinsight.ai will coordinate with
our Lab experts/Application scientist to help you out on low input samples

We have all major sequencing platforms, which include, Illumina( Novoseq X plus), PacBio-
(Revio/ONSO), Sanger sequencing platform, HPLC, GCMS platform etc.

Our wet lab scientist do visual inspection of samples on receipt and move to quality control tests
using Agarose Gel Electrophoresis (if DNA), Qubit and/or Nanodrop. Libraries are subjected to
analysis by Qubit, Tape station, Bioanalyzer and/or Pippin Pulse. Results/reports generated in each
step are shared with you for review. FastQC of Sequencing Data will also be shared for your review.

Samples are stored at -800C, -200C and 40C based on their type. DNA/RNA samples are retained up
to 3months from the receipt date, Tissue (cells/blood etc.) up to 15 days and libraries up to 2
months. If the project is not initiated within 30 days of receiving the samples, they will be discarded

Once all QC passed samples are sequenced, data delivered, and Final analysis report shared (if opted
for), your project is considered as closed. You will also receive a PCF (project closure form) which
identifies the project as closed

DATA ANALYSIS SERVICES

WHOLE GENOME SEQUENCING ANALYSIS DELIVERABLES

Standard Analysis: Raw Data QC and Report, Alignment Statistics and Report, Variation Calling Report
(SNP, In-Dels), Gene Annotation Table with Variations.

Standard Analysis: Raw Data QC & Report, Assembly Statistics and Report, Genome Finishing using
Closesthomolog, rRNA identification and analysis report,Plasmid Identification and analysis report
ProkkaAnnotation.

RE-SEQUENCING/TARGETED/EXOME:

Standard Analysis: Raw Data QC and Report, Alignment Report, Variation calling Report (SNP, In-
Dels),Basic Variation Annotation and Effect Analysis Report.

Standard Analysis: Raw Data QC and Report, Assembly Statistics and Report, Gene Prediction &
Annotation, Report.

WHOLE TRANSCRIPTOME SEQUENCING ANALYSIS DELIVERABLE​

Prokaryotic/Eukaryotic Whole Transcriptome Sequencing Analysis Deliverables

Raw Data QC and Report, Read Alignment to reference genome and transcript Identifica-tion, Quantification & Expression Profiling of transcripts, Differential Analysis among the conditions, Biolog-ical Significance Analysis of differentials. Gene ontology and Pathway enrichment analysis. Graphs such as Heatmaps, volcano plots, dispersion plot, GO and pathway enrichment graphs.

Standard Analysis: Raw Data QC & Report, Assembly Statistics and Report, Genome Finishing using
Closesthomolog, rRNA identification and analysis report,Plasmid Identification and analysis report
ProkkaAnnotation.

Standard Analysis: Sample wise Raw Data QC, Unique tags and abundance Report, Known Small RNA analysis report, Identification and Quantitation of Known miRNAs, Expression Profiling and Differential Expression Analysis of Known miRNAs. Mining of Novel miRNAs and Target Prediction

Privacy and Data use​

Your informed consent is essential before we can test you. Obtaining consent involves informing the potential test taker about his or her rights, the purpose of the test, the procedures to be undergone, and the potential risks and benefits of participation. The goal of the informed consent process is to provide sufficient information so that a potential test taker can make an informed decision about whether to take this test and further, to enrol in a study or to continue participation in the same.

This means that your DNA sample will be stored with the processing lab for a minimum period of 3 months for the purpose of conducting additional tests that you could opt-in for later.

GeneInsight values your privacy as paramount. Your demographic and personal medical data will never be shared with a third party. We may use aggregated, anonymous data from our tests as a part of our research – but there is no link to individuals in such a use of data.